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Galectin-8

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(Redirected from LGALS8)
LGALS8
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesLGALS8, Gal-8, PCTA-1, PCTA1, Po66-CBP, galectin 8
External IDsOMIM: 606099; MGI: 1928481; HomoloGene: 31386; GeneCards: LGALS8; OMA:LGALS8 - orthologs
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_006499
NM_201543
NM_201544
NM_201545

NM_001199043
NM_001291055
NM_001291057
NM_001291060
NM_018886

RefSeq (protein)

NP_006490
NP_963837
NP_963838
NP_963839

NP_001185972
NP_001277984
NP_001277986
NP_001277989
NP_061374

Location (UCSC)Chr 1: 236.52 – 236.55 MbChr 13: 12.45 – 12.48 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Galectin-8 is a protein of the galectin family that in humans is encoded by the LGALS8 gene.[5][6][7]

Function

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This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified.[7]

Galectin-8, interacts with the mTOR regulatory system composed of SLC38A9, Ragulator, RagAB, RagCD.[8] Galectin-8 controls mTOR causing its inactivation and dissociation from damaged lysosomes, hence transducing the breach of the lysosomal membrane to mTOR.[8] The physiological consequences of mTOR inhibition following lysosomal membrane damage[8] encompass autophagy and metabolic switching.

Galectin-8 levels

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Circulating galectin levels in the serum of healthy individuals typically range between 0.1 and 166.3 ng/mL, with a median concentration of approximately 6 ng/mL. In cancer patients, these levels can increase up to fivefold. [9] [10]

Role in cancer

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Galectin-8 has a complex role in cancer, sometimes being protumorigenic and other times anti-tumorigenic. [11]

Role in cellular defence

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Galectin-8 has recently been shown to have a role in cellular defence, against both bacterial cytosolic infection and vacuolar damage.[12] Many intracellular bacteria, such as S. enterica serovar Typhimurium and S. flexneri prefer to replicate inside and outside of the vacuole safety respectively, yet these vacuoles may become damaged, exposing bacteria to the host cell cytoplasm. It has been shown that the binding of galectin-8 to the damaged vacuole can recruit autophagy adaptors such as NDP52 leading to the formation of an autophagosome and subsequent bacterial destruction.[12] As knockout experiments of galectin-8 leads to more successful cytosolic replication by S. enterica serovar Typhimurium, it is thought that galectin-8 acts as a danger receptor in defence against intracellular pathogens.[12][13]

Engineered galectin-8 assays

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Galectin-8 has also been used to study endosomal disruption in the development of nanoscale drug delivery systems. Many drug delivery systems carrying large molecule drugs, such as antisense oligonucleotides, siRNA, peptides, and therapeutic proteins, are engineered to be pH-responsive, and disrupt the endosomal membrane because of the lower pH found within progressively acidifying endosomes. Galectin-8 can be tagged with a fluorophore to track these disrupted endosomal membranes, especially when coupled with automated microscopy.[14]

Interactions

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Galectin-8 has been shown to interact with CD44,[15] CD49d,[16] CD29[16] and CD49c.[16] It also interacts with components of the mTORC1 complex.[8]

References

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  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000116977Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000057554Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Hadari YR, Paz K, Dekel R, Mestrovic T, Accili D, Zick Y (February 1995). "Galectin-8. A new rat lectin, related to galectin-4". The Journal of Biological Chemistry. 270 (7): 3447–53. doi:10.1074/jbc.270.7.3447. PMID 7852431.
  6. ^ Su ZZ, Lin J, Shen R, Fisher PE, Goldstein NI, Fisher PB (July 1996). "Surface-epitope masking and expression cloning identifies the human prostate carcinoma tumor antigen gene PCTA-1 a member of the galectin gene family". Proceedings of the National Academy of Sciences of the United States of America. 93 (14): 7252–7. Bibcode:1996PNAS...93.7252S. doi:10.1073/pnas.93.14.7252. PMC 38969. PMID 8692978.
  7. ^ a b "Entrez Gene: LGALS8 lectin, galactoside-binding, soluble, 8 (galectin 8)".
  8. ^ a b c d Jia J, Abudu YP, Claude-Taupin A, Gu Y, Kumar S, Choi SW, et al. (April 2018). "Galectins Control mTOR in Response to Endomembrane Damage". Molecular Cell. 70 (1): 120–135.e8. doi:10.1016/j.molcel.2018.03.009. PMC 5911935. PMID 29625033.
  9. ^ Barrow H, Guo X, Wandall HH, Pedersen JW, Fu B, Zhao Q, et al. (November 2011). "Serum galectin-2, -4, and -8 are greatly increased in colon and breast cancer patients and promote cancer cell adhesion to blood vascular endothelium". Clinical Cancer Research. 17 (22): 7035–7046. doi:10.1158/1078-0432.CCR-11-1462. PMID 21933892.
  10. ^ Tazhitdinova R, Timoshenko AV (July 2020). "The Emerging Role of Galectins and O-GlcNAc Homeostasis in Processes of Cellular Differentiation". Cells. 9 (8): 1792. doi:10.3390/cells9081792. PMC 7465113. PMID 32731422.
  11. ^ Souchak J, Mohammed NB, Lau LS, Dimitroff CJ (2024). "The role of galectins in mediating the adhesion of circulating cells to vascular endothelium". Frontiers in Immunology. 15: 1395714. doi:10.3389/fimmu.2024.1395714. PMID 38840921.
  12. ^ a b c Thurston TL, Wandel MP, von Muhlinen N, Foeglein A, Randow F (January 2012). "Galectin 8 targets damaged vesicles for autophagy to defend cells against bacterial invasion". Nature. 482 (7385): 414–8. Bibcode:2012Natur.482..414T. doi:10.1038/nature10744. PMC 3343631. PMID 22246324.
  13. ^ Huang J, Brumell JH (February 2012). "Microbiology: A sweet way of sensing danger". Nature. 482 (7385): 316–7. Bibcode:2012Natur.482..316H. doi:10.1038/482316a. PMID 22337047. S2CID 33971618.
  14. ^ Kilchrist KV, Dimobi SC, Jackson MA, Evans BC, Werfel TA, Dailing EA, et al. (February 2019). "Gal8 Visualization of Endosome Disruption Predicts Carrier-Mediated Biologic Drug Intracellular Bioavailability". ACS Nano. 13 (2): 1136–1152. doi:10.1021/acsnano.8b05482. PMC 6995262. PMID 30629431.
  15. ^ Chakraborty A, Perez M, Carroll JD, Antonopoulos A, Dell A, Ortega L, et al. (March 2023). "Hypoxia Controls the Glycome Signature and Galectin-8-Ligand Axis to Promote Protumorigenic Properties of Metastatic Melanoma". The Journal of Investigative Dermatology. 143 (3): 456–469.e8. doi:10.1016/j.jid.2022.07.033. PMC 10123958. PMID 36174713.
  16. ^ a b c Hadari YR, Arbel-Goren R, Levy Y, Amsterdam A, Alon R, Zakut R, Zick Y (July 2000). "Galectin-8 binding to integrins inhibits cell adhesion and induces apoptosis". Journal of Cell Science. 113 (Pt 13): 2385–2397. doi:10.1242/jcs.113.13.2385. PMID 10852818.

Further reading

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