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Lucas Andrew Staehelin

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Lucas Andrew Staehelin
Born(1939-02-10)February 10, 1939
Sydney, Australia
DiedSeptember 28, 2022(2022-09-28) (aged 83)
CitizenshipSwitzerland-America
Known forCell biology, photosynthesis, electron microscopy
TitleProfessor emeritus
Academic background
Alma materSwiss Federal Institute of Technology
Academic work
InstitutionsUniversity of Colorado Boulder

Lucas Andrew Staehelin (February 10, 1939 – September 28, 2022) was a retired Swiss-American cell biologist.[1] He was professor emeritus at the University of Colorado Boulder.[2]

He developed cryofixation methods and pioneered their use for preserving cellular structures for electron microscope studies.[3][4] Application of these methods to the analysis of plant, animal and bacterial cells brought insights into the nanoscale architecture and functional organization of membranous organelles and cytoskeletal systems.[5][6][7][8] Staehelin taught undergraduate and graduate courses in cellular and molecular biology at the University of Colorado Boulder.

Early life and education

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Staehelin was born in Sydney, Australia on February 10, 1939.[citation needed] In 1948 he moved to Switzerland where he attended the Gymnasium in Bern. He studied natural sciences as an undergraduate at the Swiss Federal Institute of Technology, Zürich from 1959 to 1963, where he earned his doctorate in plant cell biology in 1966, studying in the laboratory of Kurt Mühlethaler.[9] From 1966 to 1969 he worked as a scientist at the Physics and Engineering Laboratory of the Department of Scientific and Industrial Research in New Zealand. He was a postdoctoral fellow from 1969 to 1970 at Harvard University.[citation needed][10]

Career

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Staehelin became an assistant professor in the Department of Molecular, Cellular and Developmental Biology, University of Colorado Boulder, in 1970, was promoted to associate professor in 1973, to full professor in 1978, and to professor emeritus in 2006. He held visiting professorships at Albert-Ludwigs University (1978), Swiss Federal Institute of Technology (1984, 1992), and University of Melbourne (1998).

He has authored and co-authored more than 300 scientific publications (H-Index 78).[11][12]

Research

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Staehelin's research focused on using cryofixation methods to produce improved images of cellular structures by means of freeze-fracture, thin section and immunolabeling electron microscopy, and by electron tomography techniques.[4] His studies of photosynthetic membranes led to the characterization of differences in structure and function of grana and stroma thylakoid membranes,[13] of chlorosomes,[14] and the attachment of plastoglobules to thylakoids.[15] Vitrification of plant cells using propane-jet freezing led to the discovery that cellulose fibrils were synthesized by plasma membrane-associated rosette particle complexes,[16] and the demonstration that vesicle-mediated secretion and membrane recycling in turgid cells involves characteristic, transient membrane configurations.[17] High pressure freezing of intact plant tissues led to new models of plant cytokinesis, first as seen in thin section images[18][19][7] and then by electron tomography. These studies led to the discovery of the cell plate assembly matrix within which the assembly of the cell plate from vesicles occurs by previously unknown mechanisms.[20][21] Electron tomography analysis enabled his group to produce nanoscale models of endoplasmic reticulum, Golgi apparatus and trans Golgi network membranes[22][23][24][25][4] The Golgi matrix/scaffold was shown to originate on COPII vesicles and to capture passing Golgi to mediate ER-Golgi vesicle transfer as postulated by the stop-pluck-and-go hypothesis of Golgi trafficking.[26]

Awards

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References

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  1. ^ "L. Andrew Staehelin, CU Boulder molecular biologist, dies at 83". Colorado Arts and Sciences Magazine. 5 October 2022. Retrieved 14 May 2023.
  2. ^ "Andrew Staehelin". Molecular, Cellular & Developmental Biology. 22 September 2016. Retrieved 23 March 2022.
  3. ^ Gilkey, John C.; Staehelin, L. Andrew (1986). "Advances in ultrarapid freezing for the preservation of cellular ultrastructure". Journal of Electron Microscopy Technique. 3 (2): 177–210. doi:10.1002/jemt.1060030206. ISSN 1553-0817.
  4. ^ a b c Cheung, Alice Y; Cosgrove, Daniel J; Hara-Nishimura, Ikuko; Jürgens, Gerd; Lloyd, Clive; Robinson, David G; Staehelin, L Andrew; Weijers, Dolf (15 September 2021). "A rich and bountiful harvest: key discoveries in plant cell biology". The Plant Cell. 34: 53–71. doi:10.1093/plcell/koab234. ISSN 1040-4651. PMC 8773953. PMID 34524464.
  5. ^ Nevo, Reinat; Charuvi, Dana; Tsabari, Onie; Reich, Ziv (April 2012). "Composition, architecture and dynamics of the photosynthetic apparatus in higher plants: The photosynthetic apparatus in higher plants". The Plant Journal. 70 (1): 157–176. doi:10.1111/j.1365-313X.2011.04876.x. PMID 22449050.
  6. ^ Kirchhoff, Helmut (8 March 2019). "Chloroplast ultrastructure in plants". New Phytologist. 223 (2): 565–574. doi:10.1111/nph.15730. ISSN 0028-646X. PMID 30721547. S2CID 206221619.
  7. ^ a b Jürgens, Gerd; Park, Misoon; Richter, Sandra; Touihri, Sonja; Krause, Cornelia; El Kasmi, Farid; Mayer, Ulrike (26 January 2015). "Plant cytokinesis: a tale of membrane traffic and fusion". Biochemical Society Transactions. 43 (1): 73–78. doi:10.1042/BST20140246. ISSN 0300-5127. PMID 25619248.
  8. ^ Ogbu, Chinemerem P.; Roy, Sourav; Vecchio, Alex J. (January 2022). "Disruption of Claudin-Made Tight Junction Barriers by Clostridium perfringens Enterotoxin: Insights from Structural Biology". Cells. 11 (5): 903. doi:10.3390/cells11050903. ISSN 2073-4409. PMC 8909277. PMID 35269525.
  9. ^ "Chemistry Tree - L. Andrew Staehelin".
  10. ^ "L. Andrew Staehelin". ResearchGate. Retrieved 22 March 2022.
  11. ^ "L. Andrew Staehelin, University of Colorado Boulder, Department of Molecular, Cellular, and Developmental Biology". ResearchGate. Retrieved 17 December 2021.
  12. ^ "Web of Science". www.webofscience.com. Retrieved 26 January 2022.
  13. ^ Staehelin, L. Andrew (1 April 2003). "Chloroplast structure: from chlorophyll granules to supra-molecular architecture of thylakoid membranes". Photosynthesis Research. 76 (1): 185–96. doi:10.1023/A:1024994525586. ISSN 1573-5079. PMID 16228577. S2CID 5022848.
  14. ^ Staehelin, L. Andrew; Golecki, Jochen R.; Drews, Gerhart (1 April 1980). "Supramolecular organization of chlorosomes (chlorobium vesicles) and of their membrane attachment sites in Chlorobium Limicola". Biochimica et Biophysica Acta (BBA) - Bioenergetics. 589 (1): 30–45. doi:10.1016/0005-2728(80)90130-9. ISSN 0005-2728. PMID 7356977.
  15. ^ Rottet, Sarah; Besagni, Céline; Kessler, Felix (1 September 2015). "The role of plastoglobules in thylakoid lipid remodeling during plant development". Biochimica et Biophysica Acta (BBA) - Bioenergetics. SI: Chloroplast Biogenesis. 1847 (9): 889–99. doi:10.1016/j.bbabio.2015.02.002. ISSN 0005-2728. PMID 25667966.
  16. ^ Giddings, T H Jr; Brower, D L; Staehelin, L A (1 February 1980). "Visualization of particle complexes in the plasma membrane of Micrasterias denticulata associated with the formation of cellulose fibrils in primary and secondary cell walls". Journal of Cell Biology. 84 (2): 327–339. doi:10.1083/jcb.84.2.327. ISSN 0021-9525. PMC 2110545. PMID 7189756.
  17. ^ Staehelin, L. A.; Chapman, R. L. (1 May 1987). "Secretion and membrane recycling in plant cells: novel intermediary structures visualized in ultrarapidly frozen sycamore and carrot suspension-culture cells". Planta. 171 (1): 43–57. Bibcode:1987Plant.171...43S. doi:10.1007/BF00395066. ISSN 1432-2048. PMID 24227269. S2CID 29633957.
  18. ^ Samuels, A L; Giddings, T H Jr; Staehelin, L A (15 September 1995). "Cytokinesis in tobacco BY-2 and root tip cells: a new model of cell plate formation in higher plants". Journal of Cell Biology. 130 (6): 1345–1357. doi:10.1083/jcb.130.6.1345. ISSN 0021-9525. PMC 2120572. PMID 7559757.
  19. ^ Otegui, Marisa; Staehelin, L. Andrew (1 June 2000). "Syncytial-Type Cell Plates: A Novel Kind of Cell Plate Involved in Endosperm Cellularization of Arabidopsis". The Plant Cell. 12 (6): 933–947. doi:10.1105/tpc.12.6.933. ISSN 1040-4651. PMC 149094. PMID 10852938.
  20. ^ Otegui, M. S.; Mastronarde, D. N.; Kang, B. H.; Bednarek, S. Y.; Staehelin, L. A. (September 2001). "Three-dimensional analysis of syncytial-type cell plates during endosperm cellularization visualized by high resolution electron tomography". The Plant Cell. 13 (9): 2033–2051. doi:10.1105/tpc.13.9.2033. ISSN 1040-4651. JSTOR 3871426. PMC 139450. PMID 11549762.
  21. ^ Otegui, Marisa S. (2011), Popper, Zoë A. (ed.), "Electron Tomography and Immunogold Labelling as Tools to Analyse de Novo Assembly of Plant Cell Walls", The Plant Cell Wall: Methods and Protocols, Methods in Molecular Biology, vol. 715, Totowa, NJ: Humana Press, pp. 123–140, doi:10.1007/978-1-61779-008-9_9, ISBN 978-1-61779-008-9, PMID 21222081
  22. ^ ROBINSON, DAVID G. (27 May 2020). "Plant Golgi ultrastructure". Journal of Microscopy. 280 (2): 111–121. doi:10.1111/jmi.12899. ISSN 0022-2720. PMID 32420623. S2CID 218680885.
  23. ^ Staehelin, L. Andrew; Kang, Byung-Ho (1 August 2008). "Nanoscale Architecture of Endoplasmic Reticulum Export Sites and of Golgi Membranes as Determined by Electron Tomography". Plant Physiology. 147 (4): 1454–1468. doi:10.1104/pp.108.120618. ISSN 0032-0889. PMC 2492626. PMID 18678738.
  24. ^ Kang, Byung-Ho; Nielsen, Erik; Preuss, Mary Lai; Mastronarde, David; Staehelin, L. Andrew (2011). "Electron Tomography of RabA4b- and PI-4Kβ1-Labeled Trans Golgi Network Compartments in Arabidopsis". Traffic. 12 (3): 313–329. doi:10.1111/j.1600-0854.2010.01146.x. ISSN 1600-0854. PMID 21134079. S2CID 13965499.
  25. ^ Donohoe, Bryon S.; Kang, Byung-Ho; Gerl, Mathias J.; Gergely, Zachary R.; McMichael, Colleen M.; Bednarek, Sebastian Y.; Staehelin, L. Andrew (2013). "Cis-Golgi Cisternal Assembly and Biosynthetic Activation Occur Sequentially in Plants and Algae". Traffic. 14 (5): 551–567. doi:10.1111/tra.12052. ISSN 1600-0854. PMC 3622843. PMID 23369235.
  26. ^ Nebenführ, Andreas; Gallagher, Larry A.; Dunahay, Terri G.; Frohlick, Jennifer A.; Mazurkiewicz, Anna M.; Meehl, Janet B.; Staehelin, L. Andrew (1 December 1999). "Stop-and-Go Movements of Plant Golgi Stacks Are Mediated by the Acto-Myosin System1". Plant Physiology. 121 (4): 1127–1141. doi:10.1104/pp.121.4.1127. ISSN 0032-0889. PMC 59480. PMID 10594100.
  27. ^ "Singleview - Alexander von Humboldt-Stiftung".
  28. ^ "List of Members".
  29. ^ "Historic Fellows". American Association for the Advancement of Science. Retrieved 17 December 2021.
  30. ^ a b "CU-Boulder's Andrew Staehelin Named First Ever Fellow Of American Society Of Plant Biologists". CU Boulder Today. 25 July 2007. Retrieved 17 December 2021.
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