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Maurice Green (virologist)

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Maurice Green
Maurice Green
Born(1926-05-05)May 5, 1926
New York, New York
DiedDecember 5, 2017(2017-12-05) (aged 91)
St. Louis, Missouri
NationalityAmerican
Alma materUniversity of Wisconsin, Madison (M.S., Ph.D.) University of Michigan, Ann Arbor (B.S.)
SpouseMarilyn Glick Green (1929-2010; m. 1950)
Children3
AwardsNIH R.E. Dyer Lectureship Award

1972 University of Chicago Howard Taylor Ricketts Award

1976 Burroughs-Wellcome Fellow (1986-1987)

1990 American Cancer Society John Krey III Memorial Award

American Cancer Society (Heartland Division) Spirit of Health Award

2002 Academy of Science of St. Louis Peter H. Raven Lifetime Award
Scientific career
FieldsVirology

Microbiology Biochemistry

Molecular biology
InstitutionsSt. Louis University School of Medicine
Websitehttp://medschool.slu.edu/mmi/index.php?page=maurice-green-ph-d

Maurice Green (May 5, 1926 – December 5, 2017) was an American virologist.[1] He was regarded as a pioneer in the study of animal viruses, in particular their role in cancer.[1] Green founded the Institute of Molecular Virology[2][3][4] at St. Louis University School of Medicine in the late 1950s, and later served as its chairman.

Early life

[edit]

Maurice Green was born on May 5, 1926, in New York, to Jewish parents: David Green, an emigrant from Russia, and Bessie Lipschitz, an emigrant from Lithuania. Green was the oldest of four children.[citation needed]

Following his graduation from high school in 1944, Green served in the U.S. Navy,[1] after which he earned a B.S. degree in chemistry from the University of Michigan, Ann Arbor in 1949.[5] He then received his M.S. (1952) and Ph.D. (1954)[5] degrees in biochemistry and chemistry from the University of Wisconsin, Madison. Afterwards, he pursued postdoctoral research training at the University of Pennsylvania School of Medicine, serving as an instructor of biochemistry from 1955 to 1956.

Career

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Green became a faculty member of St. Louis University School of Medicine as an assistant professor in the Department of Microbiology in 1956. He was promoted to associate professor in 1960 and then professor in 1963. In 1964, he became a professor of molecular virology and founding chairman of the Institute for Molecular Virology;[1][3][4][5] he has since held this title and position.[2]

Green was a tumor virologist, with a research career that spanned over 60 years.[1] He played a critical role in developing adenovirus as an experimental system and made many contributions to virology and molecular biology, leading to over 300 authored/co-authored publications[1] and one U.S. patent (No. 61/509,891).[6] He first coined the term molecular virology,[1] and later founded an institute bearing that name. Many of his graduate students and postdoctoral fellows have gone on to make significant contributions to the fields of virology, molecular virology, and tumor biology.[1]

Green's research in the 1960s and early 1970s was highly regarded because of the insights it provided into the role of viruses in cancer.[1][7][8][9][10][11][12]

Green received an NIH lifetime Research Career Award (1969–present),[1][5][13] the NIH R.E. Dyer Lectureship Award (1972),[5][14] and the 1976 University of Chicago Howard Taylor Ricketts Award.[5] He was a Burroughs-Wellcome Fellow (1986–1987)[citation needed] and received the 1990 American Cancer Society John Krey III Memorial Award,[citation needed] The 2002 American Cancer Society (Heartland Division) Spirit of Health Award and the 2002 Academy of Science of St. Louis Peter H. Raven Lifetime Award and Fellow.[1][5]

Research

[edit]

Green was one of the founding scientists in the field of tumor virology,[1] an area of biomedical research investigating the role that viruses play in cancer.[15][16][17][18][19][20][21][22] His early studies were major contributions to the general armamentarium of techniques and concepts used in experimental virology today.

Green conducting virology research.

In the late 1950s and early 1960s, Green was among the first scientists in the world to study biochemical features of virus replication in cell culture and to develop and apply the emerging concepts of molecular biology.[1] His major focus of investigation was human adenoviruses. He and his colleagues performed foundational viral research; growing viruses in culture, purifying virions, extracting and characterizing viral DNA,[23] and studying gene expression at the RNA and protein levels.[24] This work established the kinetics of infection, and showed that the infection was divided into two major stages of gene expression.

Green culturing virus-infected cells.

In 1962, scientists at the National Institutes of Health discovered that certain serotypes of human adenoviruses can induce tumors in newborn hamsters.[1][25] This finding was not only of scientific interest, but it also raised concerns because the military was using live adenoviruses as vaccines against adenovirus-induced acute respiratory disease. Green was asked to learn as much as he could as quickly as possible about the 31 distinct viral serotypes known at that time.[4] His studies included characterizing the viruses’ DNA, investigating the tumor-inducing properties of the viruses, and determining the molecular and kinetic parameters of adenovirus infection. He showed that adenoviruses could be divided into distinct groups based on these and other properties. These classic studies served to establish adenoviruses as a powerful model system that has since been used to address more global questions about virus replication, human cell molecular biology, infection and immunity, and neoplastic transformation. In subsequent years, the study of adenoviruses has provided key insights into tumor suppressors, cell proliferation, and the host immune response. They also emerged as a vehicle for human gene therapy[26]

Also in the early 1960s, Green and others showed that human adenoviruses could transform rodent cells in culture into a malignant state.[1][8] The mechanism behind this phenomenon was a mystery at that time. In 1966, Green published a paper in the Proceedings of the National Academy of Sciences showing for the first time that transformed cells express adenovirus-specific RNA that could be labeled in a fashion that would allow detection based on hybridization to adenovirus DNA immobilized on filters.[24] This pioneering work helped establish the principle – applicable to all tumor viruses and relevant to tumor oncogenesis more broadly – that adenoviruses transform cells via continuous expression of their genes rather than by a ‘hit-and-run’ mechanism.

In subsequent years, Green made a number of other contributions to adenovirus molecular biology. He discovered two of the proteins required for adenoviruses to transform cells, and also other proteins that usurp the infected cell and convert it into a factory for virus replication. This later work played a key role in further establishing adenovirus as an experimental system, attracting other research groups and producing many important discoveries. For example, RNA splicing was discovered using the adenovirus system by researchers at the Cold Spring Harbor Laboratory and the Massachusetts Institute of Technology (work that led to a Nobel Prize in 1989 and 1993).[27][28]

Green with long-time research partner and laboratory member, Paul Lowenstein.

In addition to determining many of the basic concepts of neurovirology, Green conducted seminal studies to determine the role that these viruses play in human cancer.[7][8][9][10][11][12] He demonstrated that although these viruses are endemic in the human population and have oncogenic potential in rodents, they appear to play no detectable part in the formation of any of the major human cancers.

Green studied not only adenoviruses but also many other tumorigenic viruses. At the time, it was commonly hypothesized that since tumor viruses cause certain cancers in animal species,[1][29][30] they might also cause cancers in humans. Green embarked on a large study to ask whether human cancers contain tumor virus genes. This study, funded by the National Institutes of Health via the Virus Cancer Program, was a major component of President Richard Nixon's ‘War on Cancer.’ Green collected over 2,500 tumor samples and analyzed them for the presence of different DNA and RNA tumor viruses. Nearly all the samples were negative. These carefully controlled data, which were published in a series of papers, argued strongly against a viral etiology of human cancer. One exception was the detection of papillomavirus DNA in urogenital cancers; Green was probably the first to make this finding, although he was the second to publish it in the scientific literature.[31]

Later, Green's research extended to the RNA tumor viruses (tumor-inducing viruses with an RNA genome). Before reverse transcriptase was discovered by David Baltimore and Howard Temin in 1970 (work that earned them a Nobel Prize), Green wrote a review predicting that the enzyme must exist in the virion of retroviruses. In the early 1970s, he and his co-workers conducted important studies on the biochemical features of reverse transcriptase of avian and murine RNA tumor viruses. These studies revealed the subunit structure of the enzyme and helped define the polymerase and Ribonuclease H activities inherent in that enzyme.[32] He coupled this basic research with the effort to detect RNA tumor viruses in human cancer. Tremendous effort was directed to the ‘simultaneous detection’ assay in which proteins are extracted from tumor samples, separated by size, and then examined for reverse transcriptase activity. There was angst associated with those studies because of the importance of the research and because the workers could occasionally detect weak activity. As was later shown, this activity was not due to infection by RNA tumor viruses, but rather to endogenous reverse transcriptase activity.[33][34] These studies were negative, again arguing against a viral etiology of cancer.

In recent years, Green has focused his studies on the multifunctional adenovirus oncoprotein E1A,[1][14][35] which is necessary for cell transformation of adenovirus non-permissive cells. He was among the first to demonstrate that individual functional domains within E1A are independent,[36] an observation that has since been exploited by other laboratories studying a number of regulatory proteins. Most recently, Green has focused on the 80-amino-acid E1A N-terminal transcriptional repression domain in order to understand the mechanism of gene control and regulation by this potent transcriptional repressor[37] he has also examined the potential application of repressing medically important genes by E1A's transcriptional repression function[38]

Green is known for founding, building, and leading the Institute for Molecular Virology[1][3][4][5] at the Saint Louis University School of Medicine. This institute and its research faculty was the site of numerous important studies on viruses, cancer, AIDS,[39][40][41][42][43] and contemporary molecular biology.

Personal life and death

[edit]

Green married Marilyn Glick on August 20, 1950. They had three children.[44][14][45]

Green died in St. Louis, Missouri, on December 5, 2017, of natural causes at the age of 91.[46]

References

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  1. ^ a b c d e f g h i j k l m n o p q r "Going Viral : Saint Louis University : SLU". www.slu.edu. Archived from the original on 2016-09-20. Retrieved 2016-07-19.
  2. ^ a b "Maurice Green, Ph.D". Saint Louis University Faculty_IMV. Archived from the original on 2016-09-20. Retrieved 2016-01-04.
  3. ^ a b c Adams, Leonard (February 27, 1966). "Cancer Research 'Dangerous'". The Pittsburgh Press. Archived from the original on May 11, 2017. Retrieved July 17, 2016 – via Google News.
  4. ^ a b c d "Cancer Studies Progress in an Old Streetcar Barn". Reading Eagle. February 27, 1966. Archived from the original on February 29, 2020. Retrieved July 17, 2016 – via Google News.
  5. ^ a b c d e f g h "University of Michigan Chemistry News Winter 2015" (PDF). University of Michigan Chemistry Department. 2015. p. 10. Archived (PDF) from the original on June 24, 2016. Retrieved July 17, 2016.
  6. ^ "United States Patent Application: 0130023481". appft.uspto.gov. Archived from the original on 2017-05-10. Retrieved 2016-07-17.
  7. ^ a b "Cancer Virus Trace Found". Spokane Daily Chronicle. April 5, 1967. Archived from the original on May 11, 2017. Retrieved July 17, 2016 – via Google News.
  8. ^ a b c Carey, Frank (April 6, 1967). "Possible Breakthrough in Determining If Some Cancers Caused by Virus". The Telegraph. Archived from the original on May 11, 2017. Retrieved July 17, 2016 – via Google News.
  9. ^ a b Spaulding, Frank (April 5, 1967). "New Technique May Show Viruses' Role in Cancer". Park City Daily News. Archived from the original on May 11, 2017. Retrieved July 17, 2016 – via Google News.
  10. ^ a b "New Break Is Seen For Cancer Job". Lawrence Journal-World. April 5, 1967. Archived from the original on February 29, 2020. Retrieved July 17, 2016.
  11. ^ a b "Cancer 'Breakthrough' Claimed by Scientist". Ellensburg Daily Record. April 5, 1967. Archived from the original on May 11, 2017. Retrieved July 17, 2016 – via Google News.
  12. ^ a b Spaulding, James (April 20, 1967). "Major Research Underway to Link Cancer to Virus". The Milwaukee Journal. Retrieved July 17, 2016 – via Google News.
  13. ^ "A 44-year-old grant | The Scientist Magazine®". The Scientist. Archived from the original on 2017-05-11. Retrieved 2016-07-17.
  14. ^ a b c MacDougall, Raymond (October 3, 2008). "Biomedical Research: It's In the Greens". NIH Record. Archived from the original on May 10, 2017. Retrieved July 17, 2016.
  15. ^ Baker, Carl (1975). An Administrative History of the National Cancer Institute's Viruses and Cancer Programs. National Institutes of Health – via history.nih.gov.
  16. ^ Kalland, Karl-Henning; Ke, Xi-Song; Øyan, Anne Margrete (2009-05-01). "Tumour virology--history, status and future challenges". APMIS. 117 (5–6): 382–399. doi:10.1111/j.1600-0463.2009.02452.x. ISSN 1600-0463. PMID 19400863. S2CID 41979414.
  17. ^ Olson, James Stuart (1989). The History of Cancer: An Annotated Bibliography. ABC_CLIO. ISBN 0313258899.
  18. ^ Kelly, Elizabeth; Russell, Stephen J. (2007-04-01). "History of oncolytic viruses: genesis to genetic engineering". Molecular Therapy. 15 (4): 651–659. doi:10.1038/sj.mt.6300108. ISSN 1525-0016. PMID 17299401.
  19. ^ Morange, Michael (1997). "From the Regulatory Vision of Cancer to the Oncogene Paradigm, 1975–1985". Journal of the History of Biology. 30 (1): 1–29. doi:10.1023/A:1004255309721. PMID 11618978. S2CID 6033997.
  20. ^ Marcum, J. (2002). From Heresy to Dogma in Accounts of Opposition to Howard Temin's DNA Provirus Hypothesis. History and Philosophy of the Life Sciences, 24(2), 165-192. Retrieved from JSTOR 23332384
  21. ^ Richards, Victor (1978-01-01). Cancer: The Wayward Cell : Its Origins, Nature, and Treatment. University of California Press. ISBN 9780520035966.
  22. ^ Doerfler, W. (2012-12-06). The Molecular Biology of Adenoviruses I: 30 Years of Adenovirus Research 1953–1983. Springer Science & Business Media. ISBN 9783642694608.
  23. ^ Green, M.; Pina, M. (1963-07-01). "Similarity of DNAs isolated from tumor-inducing viruses of human and animal origin". Proceedings of the National Academy of Sciences. 50 (1): 44–46. Bibcode:1963PNAS...50...44G. doi:10.1073/pnas.50.1.44. ISSN 0027-8424. PMC 300651. PMID 13950094.
  24. ^ a b Green, Maurice; Fujinaga, K (1966). "The mechanism of viral carcinogenesis by DNA mammalian viruses: viral-specific RNA in polyribosomes of adenovirus tumor and transformed cells". Proceedings of the National Academy of Sciences. 55 (6): 1567–74. Bibcode:1966PNAS...55.1567F. doi:10.1073/pnas.55.6.1567. PMC 224360. PMID 5227675.
  25. ^ HUEBNER (1963). "Specific Adenovirus Complement-Fixing Antigens in Virus-Free Hamster and Rat Tumors". Proceedings of the National Academy of Sciences. 50 (2): 379–389. Bibcode:1963PNAS...50..379H. doi:10.1073/pnas.50.2.379. PMC 221184. PMID 14060660.
  26. ^ Wold, William S. M.; Toth, Karoly (2013-12-01). "Adenovirus vectors for gene therapy, vaccination and cancer gene therapy". Current Gene Therapy. 13 (6): 421–433. doi:10.2174/1566523213666131125095046. ISSN 1875-5631. PMC 4507798. PMID 24279313.
  27. ^ "The Nobel Prize in Physiology or Medicine 1993". www.nobelprize.org. Archived from the original on 2016-05-17. Retrieved 2016-05-22.
  28. ^ "The Nobel Prize in Physiology or Medicine for 2001 - Press Release". www.nobelprize.org. Archived from the original on 2017-10-15. Retrieved 2016-05-22.
  29. ^ "Clocks and maps are needed in development". New Scientist. 1973. Archived from the original on August 10, 2023. Retrieved July 17, 2016 – via Google Books.
  30. ^ Gould, Donald (1964). "Hunting the leukaemia virus". New Scientist. No. 412. pp. 89–91. Archived from the original on August 10, 2023. Retrieved July 17, 2016 – via Google Books.
  31. ^ Green, M.; Brackmann, K. H.; Sanders, P. R.; Loewenstein, P. M.; Freel, J. H.; Eisinger, M.; Switlyk, S. A. (1982). "Isolation of a human papillomavirus from a patient with epidermodysplasia verruciformis: Presence of related viral DNA genomes in human urogenital tumors". Proceedings of the National Academy of Sciences. 79 (14): 4437–4441. Bibcode:1982PNAS...79.4437G. doi:10.1073/pnas.79.14.4437. PMC 347470. PMID 6289302.
  32. ^ Grandgenett, D. P.; Gerard, G. F.; Green, M. (1973). "A Single Subunit from Avian Myeloblastosis Virus with Both RNA-Directed DNA Polymerase and Ribonuclease H Activity". Proceedings of the National Academy of Sciences. 70 (1): 230–234. Bibcode:1973PNAS...70..230G. doi:10.1073/pnas.70.1.230. PMC 433221. PMID 4119223.
  33. ^ Gallo, Robert (1991). Virus Hunting: AIDS, Cancer, and the Human Retrovirus : a Story of Scientific Discovery. Basic Books. pp. 75. ISBN 0465098150 – via Google Books.
  34. ^ Chedd, Graham (1971). "RNA to DNA: a revolution in reverse". New Scientist. Archived from the original on August 10, 2023. Retrieved July 17, 2016 – via Google Books.
  35. ^ "Biochemistry of Viral Replication". 360 BIO. September 1, 1977. Archived from the original on December 20, 2016. Retrieved July 17, 2016.
  36. ^ Lillie, J. W.; Loewenstein, P. M.; Green, M. R.; Green, M. (1987). "Functional domains of adenovirus type 5 E1a proteins". Cell. 50 (7): 1091–1100. doi:10.1016/0092-8674(87)90175-9. PMID 2957064. S2CID 23602247.
  37. ^ Loewenstein, P. M., Wu, S., Chiang, C., & Green, M. (2012). The adenovirus E1A N-terminal repression domain represses transcription from a chromatin template in vitro. Virology, 428
  38. ^ Loewenstein, P. M.; Green, M. (2011). "Expression of the Adenovirus Early Gene 1A Transcription-Repression Domain Alone Downregulates HER2 and Results in the Death of Human Breast Cancer Cells Upregulated for the HER2 Proto-Oncogene". Genes & Cancer. 2 (7): 737–744. doi:10.1177/1947601911426570. PMC 3218411. PMID 22207899.
  39. ^ "St. Louis researchers uncover key scientific discoveries about AIDS". The Southeast Missourian. December 25, 1988. Archived from the original on May 11, 2017. Retrieved July 17, 2016 – via Google News.
  40. ^ Haney, Daniel (July 14, 1989). "Mutant Proteins could be cure for AIDS". Spokane Chronicle. Archived from the original on May 11, 2017. Retrieved July 17, 2016 – via Google News.
  41. ^ Haney, Daniel (July 14, 1989). "Proteins may halt the spread of AIDS". The Telegraph. Archived from the original on May 11, 2017. Retrieved July 17, 2016 – via Google News.
  42. ^ "Discovery may stop the AIDS virus". New Straits Times. July 15, 1989. Archived from the original on May 11, 2017. Retrieved July 17, 2016 – via Google News.
  43. ^ Hanely, Daniel (July 14, 1989). "Fake Proteins Can Jam AID Virus, Docs Say". Schenectady Gazette. Archived from the original on May 11, 2017. Retrieved July 17, 2016 – via Google News.
  44. ^ Powers, Ellia (November 5, 2010). "Genome research institute director is part of St. Louis' first family of science". Saint Louis Beacon. Archived from the original on July 20, 2013. Retrieved July 17, 2016.
  45. ^ "Ladue Alumnus, National Human Genome Research Institute Director Speaks with Students". Spotlight on Ladue Schools. Ladue Schools. September 18, 2015. Archived from the original on August 18, 2016. Retrieved July 17, 2016.
  46. ^ "Maurice Green, pioneering virus researcher at SLU, dies at 91". St. Louis Post-Dispatch. 8 December 2017. Archived from the original on 9 December 2017. Retrieved December 10, 2017.
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