Talk:MCherry

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Molecular Biology: MCB

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Wiki Education Foundation-supported course assignment

This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available on the course page. Student editor(s): RachelMiller8, ATouchton94. Peer reviewers: Jonathanb150, Jamezdavis2014.

Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 00:22, 18 January 2022 (UTC)[reply]

Jonathan Bentata's Review:

mCherry emits light 'with in the range' --> I would say 'in the range' Why is mCherry more stable than other fluorophores? I think you can expand a little more The section Uses is a little unclear, I would be more straight forward and try to convey in a simpler manner how mCherry is used and its mechanism. After reading this section I still do not understand mCherry mechanism, so I think you could improve that. Also, maybe include a photo showing mechanism like they show in the paper we read for Activity #2. who discover mCherry, how?--> maybe add at the beggining. Also you could add the importance of this discovery I think the 'Structure' section is pretty clear! I would just mention the 3 aminoacids that make up the chromophore if you know them Good job!

Jamez Davis' Review:

mCherry section:

Clean up sentence structures

"...from DsRed from Discosma sea..." --> "from DsRed of Discosma sea..."

"Genome editing...into the genome of many diverse.." --> "Genome editing... into the (cells/genetic code/genetic material/chromosomes) of many diverse..."

Second to last sentence of this section is a little hard to understand. I suggest making it clearing why the comparison study should be performed in vitro and what this means the results of this comparison shows.

Include commas in the last sentence ("mRFPs, like mCherry, are...")

Expand more on the usefulness of mRFPs relative to mCherry based on their sizes

Development section:

Define "maturation rate"

Suggest speaking more in present tense (instead of "was" use "is" when appropriate)

Clean up sentence structures

"...was derived from DsRed was a monomer..." --> "...is derived from DsRed and is a monomer, so it is smaller, but..."

"mCherry and other mFruits...over both DsRed and mRFP1 mCherry was developed..." --> "mCherry and other mFruits...over both DsRed and mRFP1. mCherry was developed..." (Include a "." to reduce run-on into two sentences)

The fifth sentence, "The mFruits in general were...", should be re-written to be more comprehensive demonstrating why exactly mFruits were developed and why requiring similar tetramers is an issue.

"mCherry out of all of the true monomers developed has the longest" --> "mCherry, out of all of the true monomers developed, has the longest"

Explain what it means to have a low quantum yield

Structure section:

Clean up sentence structures

"The number of these residues in sequence at 66, 67, and 68..." --> "The number of these residues in sequence are 66, 67, and 68..."

"...than the environment around the chromophore is DsRed is." --> "...than the environment around the chromophore of DsRed."

Overall comments:

Overall, the explanation of the topic is well described and proper citation is included validating the facts you present. My only comment would be to go through the entire page and address grammatical errors and be sure to explain the subject in the present tense, as to not confuse the reader about the validity of the facts. Great job and keep up the good work. Happy editing!

Jamezdavis2014 (talk) 06:27, 13 November 2018 (UTC)[reply]

Name explained

The m in mCherry should be explained. I assume cherry refers to the colour, but the m part? 2A02:8388:1604:F600:0:0:0:4 (talk) 12:31, 16 October 2021 (UTC)[reply]