User:Chh38430/Acinetobacter baylyi

A. baylyi under 10x ocular lens and 100x objective lens with crystal violet stain.

History of A. baylyi

Discovery

A. baylyi was first discovered in activated sludge of Victoria, Australia alongside six other new Acinetobacter species. A. baylyi is named after Ronald Bayly, an Australian microbiologist.

A. baylyi is a strain of the genus Acinetobacter. Acinetobacter was first discovered in 1911 by M.W. Beijerinck, a Dutch microbiologist. Acinetobacter was considered to be a well-investigated genus by the 1990s. However, only nine species were named by the early 2000s.

Characteristics

Characteristics

• strict aerobe

Biology and Biochemistry

Metabolism

A. baylyi metabolic pathways have been used for many studies in microbial metabolism. This is due to the utilization of different compounds for metabolic processes. The use of D-Asp and L-Asp in A. baylyi is an example of this metabolic tolerance for different compounds. A. baylyi is able to use both of these as carbon sources and thus opens the door to see how d enantiomers can be used for bacterial growth. Use of A. baylyi's proteins in heterologous expression inside of E. coli allowed for the bacteria to use the D-enantiomer of Asp as a carbon source.

A. Baylyi in the Laboratory

Multiple characteristics and the genetic makeup of A. baylyi constitute it to be an ideal bacterium used in experiments. A. baylyi is known for its fast growth rate and ability to be easily cultured. This bacteria is also versatile in its nutrition and is well known within the scientific community. A. baylyi is also known as an omnipresent bacteria meaning it can be found many places in nature. A. baylyi, specifically the strain ADP1, has been used for over a quarter of a century in several molecular biology studies. For these reasons, A. baylyi is used in multiple laboratory settings including genetic mechanisms such as gene duplication and amplification and bacterial metabolism.

Genetic Mechanisms

A major mechanism of A. baylyi is the ability for genetic amplification and incorporation of foreign DNA. Through experiments, A. baylyi has demonstrated how using various forms of recombination, can allow the microbe to do transcription and translation of foreign DNA. This can have major application in the genetic modification realm of microbiology as well as production of antibiotics. This is done by the mechanisms homologous, homologous facilitated illegitimate, double illegitimate, and homologous facilitated double illegitimate recombination of DNA in the genome.

Simplified version of genetic mechanisms:

• one of a.baylyi's major characteristics is that it can easily take in foreign DNA.
• it does this by recombination (add hyperlink of recomb. page here), which is the crossing over of chromosomes during meiosis.
• the ease at which a.baylyi is able to take in foreign DNA is beneficial to its longevity. because of this, a.baylyi largely contributes to antibiotic resistance. these traits make a.baylyi an ideal microbe for laboratory experiments.

One major characteristic of A. baylyi is its ability to take in foreign DNA. It does so by recombination, which is the crossing over of chromosomes during meiosis. The ease at which A. baylyi can take in foreign DNA is beneficial to its longevity, as the intake of foreign DNA contributes greatly to antibiotic resistance. This makes A. baylyi an ideal microbe for laboratory experiments.