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VPS25

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VPS25
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesVPS25, DERP9, EAP20, FAP20, vacuolar protein sorting 25 homolog
External IDsOMIM: 610907; MGI: 106354; HomoloGene: 6303; GeneCards: VPS25; OMA:VPS25 - orthologs
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_032353

NM_001284411
NM_001284412
NM_001284414
NM_026776

RefSeq (protein)

NP_115729
NP_115729.1

NP_001271340
NP_001271341
NP_001271343
NP_081052

Location (UCSC)Chr 17: 42.77 – 42.78 MbChr 11: 101.14 – 101.15 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse
ESCRT-II complex subunit
crystal structure of subunit vps25 of the endosomal trafficking complex escrt-ii
Identifiers
SymbolESCRT-II
PfamPF05871
InterProIPR008570
Available protein structures:
Pfam  structures / ECOD  
PDBRCSB PDB; PDBe; PDBj
PDBsumstructure summary

Vacuolar protein-sorting-associated protein 25 is a protein that in humans is encoded by the VPS25 gene.[5][6]

It is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT (ESCRT-I, -II, -III) complexes orchestrate efficient sorting of ubiquitinated transmembrane receptors to lysosomes via multivesicular bodies (MVBs).[7] ESCRT-II recruits the transport machinery for protein sorting at MVB.[8] In addition, the human ESCRT-II has been shown to form a complex with RNA polymerase II elongation factor ELL in order to exert transcriptional control activity. ESCRT-II transiently associates with the endosomal membrane and thereby initiates the formation of ESCRT-III, a membrane-associated protein complex that functions immediately downstream of ESCRT-II during sorting of MVB cargo. ESCRT-II in turn functions downstream of ESCRT-I, a protein complex that binds to ubiquitinated endosomal cargo.[9]

ESCRT-II is a trilobal complex composed of two copies of vps25, one copy of vps22 and the C-terminal region of vps36. The crystal structure of vps25 revealed two winged-helix domains, the N-terminal domain of vps25 interacting with vps22 and vps36.[10]

References

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  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000131475Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000078656Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Yorikawa C, Shibata H, Waguri S, Hatta K, Horii M, Katoh K, Kobayashi T, Uchiyama Y, Maki M (Mar 2005). "Human CHMP6, a myristoylated ESCRT-III protein, interacts directly with an ESCRT-II component EAP20 and regulates endosomal cargo sorting". Biochem J. 387 (Pt 1): 17–26. doi:10.1042/BJ20041227. PMC 1134928. PMID 15511219.
  6. ^ "Entrez Gene: VPS25 vacuolar protein sorting 25 homolog (S. cerevisiae)".
  7. ^ Gill DJ, Teo H, Sun J, Perisic O, Veprintsev DB, Emr SD, Williams RL (January 2007). "Structural insight into the ESCRT-I/-II link and its role in MVB trafficking". EMBO J. 26 (2): 600–12. doi:10.1038/sj.emboj.7601501. PMC 1783442. PMID 17215868.
  8. ^ Teo H, Perisic O, Gonzalez B, Williams RL (October 2004). "ESCRT-II, an endosome-associated complex required for protein sorting: crystal structure and interactions with ESCRT-III and membranes". Dev. Cell. 7 (4): 559–69. doi:10.1016/j.devcel.2004.09.003. PMID 15469844.
  9. ^ Babst M, Katzmann DJ, Snyder WB, Wendland B, Emr SD (August 2002). "Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body". Dev. Cell. 3 (2): 283–9. doi:10.1016/S1534-5807(02)00219-8. PMID 12194858.
  10. ^ Wernimont AK, Weissenhorn W (December 2004). "Crystal structure of subunit VPS25 of the endosomal trafficking complex ESCRT-II". BMC Struct. Biol. 4 (1): 10. doi:10.1186/1472-6807-4-10. PMC 539351. PMID 15579210.

Further reading

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This article incorporates text from the public domain Pfam and InterPro: IPR008570